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Quick Details

  • CAS No.: 9031-11-2
  • Other Names: Betagalactosidase
  • EINECS No.: 215-807-5
  • Place of Origin: Italy
  • Type: Enzyme Preparations

Specifications

Powder lactase in various formulations suitable for dairy applications as for food supplement.
Product Lyophilized. Food grade

Description
LactoDigest F 100K is a ß-galactosidase produced by the fermentation of a non-genetically modified strain of
Aspergillus oryzae. The lactase hydrolyzes the ß-1,4-glycosidic bonds (linkages) in lactose; releasing an equal molar
mixture of glucose and galactose. The food grade powder contains no chemical preservatives.
Astrolact Digest F100k:
• Selectively and efficiently hydrolyzes the lactose in milk and dairy products to glucose and galactose:
• Relieving the symptoms of lactose intolerance
• Increase sweetness and depress the freezing point
• Preventing lactose crystallization and its negative impact on mouth feel and protein stability
• Is active, stable and effective at low pH.
• Is a high activity lactase that provides advantages in high dose, formulations including encapsulation and
tableting.
• Is standardized to ensure process reproducibility and efficiencies, and cost/performance.
• Is standardized to ensure process reproducibility and efficiencies, and cost/performance.
• Complies with FCC and FAO/WHO JECFA recommended specifications for food grade enzymes

Usage
Astrolact Digest F100k is applicable for the hydrolysis of lactose in low pH, acidic milk and dairy products (pH 3.5-
5.5), in treatments for lactose intolerance, and in the formulation of digestive aids and supplements.
Enzyme requirements and performance are generally dictated by the physical and chemical properties of the
substrate, the substrate concentration, the desired degree of hydrolysis, and the catalytic environment (Temperature,
pH, reaction time, etc.). In consideration of your process and processing conditions, or the diet of the targeted market,
conduct tests to establish and optimize use.
In dietary aids and supplement applications initially evaluate Astrolact Digest F100k:
• 90 mg (9,000 FCC LacU) per dose
For the hydrolysis of lactose in milk and dairy products initially evaluate Astrolact Digest F100k:
• 0.10-0.20% based on the dry weight of lactose [1.0-2.0 kg/metric ton lactose dry solids basis (DSB)].
Just before use, dissolve one (1) part Astrolact Digest F100k Just before use, dissolve one (1) part in nine (9) parts
potable water with gentle agitation. Add the enzyme solution to the reaction vessel with continuous agitation to
ensure homogeneous enzyme distribution in the lactose substrate solution.
In general, a higher enzyme dose will be required when reaction temperatures are lower or when the reaction time is
decreased:
1. The rate of substrate (lactose) hydrolysis is proportional to the enzyme concentration when reaction
conditions are optimal and the substrate concentration is in excess. In such cases, doubling the enzyme dose
will hydrolyze an equivalent quantity of substrate in one-half the time or twice the quantity of substrate in
the same period of time.
2. Up to the temperature of enzyme denaturation and inactivation a higher enzyme dose will be required when
process temperatures are lower or when the reaction time is decreased. In such cases, decreasing the
process temperature 10°C (18°F) or shortening the reaction time onehalf will necessitate doubling the
enzyme dose. Conversely, increasing the process temperature 10°C (18°F) or doubling the reaction time will
decrease the enzyme dose one-half.

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